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Note: the color bars in following pictures are addedd for illustration purpose

A. Saving and loading the chimera session

1. Click on "Downloads" on the right side of the screen.
2. Navigate to the desired file e.g. chimera session file for conservation/specialization, and click on the "download" link.
3. Unzip the session file.
4. Open chimera session by double click the file name from window or you can use the File -> Open on the Menu-Bar. Alternatively, you can open session files from command line

B. Interpreting the strucutre:

1. Chimera session file for conservation will display the colours of the corresponding regions of the structure showing the conservation across all groups in the alignment.

Fig 1. Displaying the conservation regions across all groups in chimera session



2. Chimera session file for specialization will display the colours of the corresponding regions of the structure showing the positions that are "conserved but different" (specific) across all groups.

Fig 2. Displaying the specificity regions across all groups in chimera session